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Title 

Effect of Ca2+ on the activity and structure of α-glucosidase: Inhibition kinetics and molecular dynamics simulations

Authors 

X ZhangL ShiX LiQ ShengL YaoD ShenZ R LuH M ZhouY D ParkJinhyuk LeeQ Zhang

Publisher 

Elsevier

Issue Date 

2014

Citation 

Journal of Bioscience and Bioengineering, vol. 117, no. 6, pp. 696-705

Keywords 

ANSInhibition kineticsMolecular dynamicsPNPPNPGSimulationα-Glucosidase

Abstract 

Understanding the mechanism of inhibition of α-glucosidase (EC 3.2.1.20) is clinically important because of the involvement of this enzyme in type 2 diabetes mellitus. In this study, we conducted inhibition kinetics of α-glucosidase with Ca2+ and 10-ns molecular dynamics simulations. We found that direct binding of Ca2+ to the enzyme induced structural changes and inhibited enzyme activity. Ca2+ inhibited α-glucosidase in a mixed-type reaction (Ki=27.0±2.0mM) and directly induced the unfolding of α-glucosidase, which resulted in the exposure of hydrophobic residues. The simulations suggest that thirteen Ca2+ ions may interact with α-glucosidase residues and that the Ca2+ binding sites are associated with the structural changes in α-glucosidase. Our study provides insight into the mechanism of the Ca2+-induced structural changes in α-glucosidase and the inhibition of ligand binding. These results suggest that Ca2+ could act as a potent inhibitor of α-glucosidase for the treatment of type 2 diabetes mellitus.

ISSN 

1389-1723

Link 

http://dx.doi.org/10.1016/j.jbiosc.2013.12.003

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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