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Title 

Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry

Authors 

Eun Young KimJi Won KimWon Kon KimBaek Soo HanSung Goo ParkBong Hyun ChungSang Chul LeeKwang-Hee Bae

Publisher 

Public Library of Science

Issue Date 

2014

Citation 

Plos One, vol. 9, no. 5, pp. e97747-e97747

Abstract 

Background: Adipose tissue, mainly composed of adipocytes, plays an important role in metabolism by regulating energy homeostasis. Obesity is primarily caused by an abundance of adipose tissue. Therefore, specific targeting of adipose tissue is critical during the treatment of obesity, and plays a major role in overcoming it. However, the knowledge of cell-surface markers specific to adipocytes is limited. Methods and Results: We applied the CELL SELEX (Systematic Evolution of Ligands by EXponential enrichment) method using flow cytometry to isolate molecular probes for specific recognition of adipocytes. The aptamer library, a mixture of FITC-tagged single-stranded random DNAs, is used as a source for acquiring molecular probes. With the increasing number of selection cycles, there was a steady increase in the fluorescence intensity toward mature adipocytes. Through 12 rounds of SELEX, enriched aptamers showing specific recognition toward mature 3T3-L1 adipocyte cells were isolated. Among these, two aptamers (MA-33 and 91) were able to selectively bind to mature adipocytes with an equilibrium dissociation constant (Kd) in the nanomolar range. These aptamers did not bind to preadipocytes or other cell lines (such as HeLa, HEK-293, or C2C12 cells). Additionally, it was confirmed that MA-33 and 91 can distinguish between mature primary white and primary brown adipocytes. Conclusions: These selected aptamers have the potential to be applied as markers for detecting mature white adipocytes and monitoring adipogenesis, and could emerge as an important tool in the treatment of obesity.

ISSN 

1932-6203

Link 

http://dx.doi.org/10.1371/journal.pone.0097747

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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