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Title 

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Authors 

Hyun A KimSuk Yoon KwonM S YangP S Choi

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2014

Citation 

Journal of Plant Biotechnology, vol. 41, no. 1, pp. 50-55

Keywords 

Dengue virusEdible vaccineImmature embryoMaize transformationParomomycin

Abstract 

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2014.41.1.50

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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