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Title 

Label-free measurement of cell viability via counting cells attached on affinity substrates

Authors 

Junhyoung AhnJina ParkYeon-Gu KimEun Gyo LeeM G KimYong Beom Shin

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Biotechnology and Bioprocess Engineering, vol. 19, no. 2, pp. 257-261

Keywords 

affinity bindingcell attachmentcell viabilityCHO celllabel-free measurement

Abstract 

The commonly used trypan blue dye exclusion method and other modified cell viability methods, such as fluorescein dye and tetrazolium dye exclusion, artificially introduce toxic chemicals to cells and, thus, alter cellular organelles when measuring cell viability. Therefore, cell viability could be affected by the processes currently used to observe viability. In this study, the cell viability of Chinese hamster ovary (CHO) cells was measured by simply counting attached cells after the cultured CHO cells were attached on a Concanavalin A (Con A) substrate. The efficiency of cell attachment to Con A surfaces was different for live and dead cells allowing the cell viability of CHO cells to be measured without any chemical modifications to the cells.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-013-0556-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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