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Title 

Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene

 

택사디엔의 생산증가를 위한 Nicotiana benthamiana 대사공학

Authors 

M M HasanHyun Soon KimJae Heung JeonS H KimB MoonJ Y SongS H ShimK H Baek

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Plant Cell Reports, vol. 33, no. 6, pp. 895-904

Keywords 

Metabolic engineeringMetabolic pathway shuntingMethyl jasmonateTaxadiene synthaseTaxol

Abstract 

Key message: We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol®) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been used extensively to treat various types of cancer. Taxadiene, the first committed product of paclitaxel synthesis is cyclized from geranylgeranyl diphosphate (GGPP), and further complex hydroxylation and acylation processes of the unique taxane core skeleton produce paclitaxel. To accomplish de novo production of taxadiene, we transformed Nicotiana benthamiana with a taxadiene synthase (TS) gene. The introduced TS gene under the transcriptional control of the CaMV 35S promoter was constitutively expressed in N. benthamiana, and the de novo production of taxadiene was confirmed by mass spectroscopy profiling. Transformed N. benthamiana homozygous lines produced 11-27 μg taxadiene/g of dry weight. The highest taxadiene production line TSS-8 was further treated with an elicitor, methyl jasmonate, and metabolic pathway shunting by suppression of the phytoene synthase gene expression which resulted in accumulation of increased taxadiene accumulation by 1.4- or 1.9-fold, respectively. In summary, we report that the production of taxadiene in N. benthamiana was possible by the ectopic expression of the TS gene, and higher accumulation of taxadiene could be achieved by elicitor treatment or metabolic pathway shunting of the terpenoid pathway.

ISSN 

0721-7714

Link 

http://dx.doi.org/10.1007/s00299-014-1568-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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