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Title 

Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress

Authors 

Seung-Bin YoonSeon-A ChoiBo Woong SimJi-Su KimSeong-Eun MunPil-Soo JeongHae-Jun YangYoungjeon LeeYoungho ParkBong Seok SongYoung-Hyun KimKang Jin JeongJae Won HuhSang-Rae LeeSun-Uk KimKyu Tae Chang

Publisher 

Society for the Study of Reproduction

Issue Date 

2014

Citation 

Biology of Reproduction, vol. 90, no. 5, pp. 104-113

Keywords 

early developmentembryo cultureendoplasmic reticulum stressreactive oxygen species

Abstract 

The stress produced by the coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) has been explored extensively, but little is known regarding their roles in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared with the tension produced by 5% O2, 20% O2 significantly decreased the blastocyst formation rate and cell survival, which was accompanied by increases in ROS and in levels of sXBP-1 transcript, which is an ER stress indicator. In addition, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment in developing bovine embryos. Consistent with this observation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst developmental rate, trophectoderm proportion, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation with TUDCA, suggesting a possible mechanism governing the mutual regulation between ROS and ER stress. Interestingly, knockdown of XBP-1 transcripts resulted in both elevation of ROS and decrease of antioxidant transcripts, which ultimately reduced in vitro developmental competence of bovine embryos. Based on these results, in vitro developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stresses. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.

ISSN 

0006-3363

Link 

http://dx.doi.org/10.1095/biolreprod.113.113480

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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