상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Simultaneous quantitation and validation of triterpenoids and phytosteroids in Phaseolus angularis seeds

Authors 

Ju Tae HwangHyun-Mee OhMi Hwa KimHyung Jae JeongChul LeeHyun Jae JangSeung Woong LeeChan Sun ParkMun Chual Rho

Publisher 

MDPI

Issue Date 

2014

Citation 

Molecules, vol. 19, no. 7, pp. 10309-10319

Keywords 

HPLC-UVPhaseolus angularis seedsTLR4Validation

Abstract 

A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%-100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1-4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 μM.

ISSN 

1420-3049

Link 

http://dx.doi.org/10.3390/molecules190710309

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


Files in This Item: SizeFormat
12430.pdf246KbAdobe PDF
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)