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Title 

RNA-guided genome editing in Drosophila with the purified Cas9 protein

Authors 

Jeong Soo LeeSu-Jin KwakJ KimAe Kyung KimHae Min NohJ S KimKweon Yu

Publisher 

Genetics Society of America

Issue Date 

2014

Citation 

G3, vol. 4, no. 7, pp. 1291-1295

Keywords 

Cas9 proteinDrosophilaRGEN

Abstract 

We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.

ISSN 

2160-1836

Link 

http://dx.doi.org/10.1534/g3.114.012179

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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