상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Identification of lactoferrin as a human dedifferentiation factor through the studies of reptile tissue regeneration mechanisms

Authors 

K S BaeSun Young KimS Y ParkA J JeongH H LeeJungwoon LeeYee Sook ChoS H LeemT H KangKwang-Hee BaeJ H KimY W JungW JunSuk Ran YoonSang Chul LeeJ W Chung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2014

Citation 

Journal of Microbiology and Biotechnology, vol. 24, no. 6, pp. 869-878

Keywords 

DedifferentiationIPSCsLactoferrinStem cellTissue regeneration

Abstract 

In this study, we performed two-dimensional electrophoresis with protein extracts from lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homolog of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation, at least partly by increasing the expression of Klf4.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1402.02009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)