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Title 

Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high-level production of L-2,3-butanediol

Authors 

Jang Min ParkWon-Kyung HongSung Mok LeeSun-Yeon HeoYou Ree JungIn Yeong KangBaek Rock OhJeong-Woo SeoChul Ho Kim

Publisher 

Springer

Issue Date 

2014

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 41, no. 9, pp. 1425-1433

Keywords 

2,3-Butanediol dehydrogenase/acetoin reductaseKlebsiella pneumoniaL-2,3-butanediolStereospecificity

Abstract 

Klebsiella pneumoniae synthesize large amounts of L-2,3-butanediol (L-2,3-BD), but the underlying mechanism has been unknown. In this study, we provide the first identification and characterization of an L-2,3-BD dehydrogenase from K. pneumoniae, demonstrating its reductive activities toward diacetyl and acetoin, and oxidative activity toward L-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Synthesis of L-2,3-BD was remarkably enhanced by increasing gene dosage, reaching levels that, to the best of our knowledge, are the highest achieved to date.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1007/s10295-014-1483-7

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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