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Title 

Luteolin induces cell cycle arrest and apoptosis through extrinsic and intrinsic signaling pathways in MCF-7 breast cancer cells

Authors 

S H ParkS HamT H KwonM S KimD H LeeJ W KangSei-Ryang OhD Y Yoon

Publisher 

Begell House

Issue Date 

2014

Citation 

Journal of Environmental Pathology, Toxicology and Oncology, vol. 33, no. 3, pp. 219-231

Keywords 

ApoptosisBreast cancerCell cycle arrestDR5Luteolin

Abstract 

Luteolin is a common flavonoid that exists in medicinal herbs, fruits, and vegetables. Luteolin has biochemical functions including anti-allergy, anti-inflammation, and anti-cancer functions. However, its efficacy and precise mode of action against breast cancer are still under study. To elucidate whether luteolin exhibits an anticancer effect in breast cancer, MCF-7 breast cancer cells were incubated with luteolin, and apoptosis was assessed by observing nuclear morphological changes and by performing cell viability assay, cell cycle analysis, annexin V-FITC/PI double staining, western blotting, RT-PCR, and mitochondrial membrane potential measurements. Luteolin inhibited growth through perturbation of cell cycle progression at the sub-G1 and G1 phases in MCF- 7 cells. Furthermore, luteolin enhanced the expression of death receptors, such as DR5, and activated caspase cascades. It enhanced the activities of caspase-8/-9/-3 in a dose-dependent manner, followed by inactivation of PARP. Activation of caspase-8 and caspase-9 induced caspase-3 activity, respectively, in apoptosis of extrinsic and intrinsic pathways. Luteolin also induced mitochondrial membrane potential collapse and cytochrome c release, and increased Bax expression by inhibiting expression of Bcl-2. Taken together, these results suggest that luteolin provokes cell cycle arrest and induces apoptosis by activating the extrinsic and intrinsic pathways.

Citation 

Journal of Environmental Pathology, Toxicology and Oncology, 33(3): 219-231

ISSN 

0731-8898

Link 

http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2014010923

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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