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Title 

Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting

Authors 

Y J BaeK M ParkS H AhnJeong Hee MoonM S Kim

Publisher 

Elsevier

Issue Date 

2014

Citation 

Journal of American Society for Mass Spectrometry, vol. 25, no. 8, pp. 1502-1505

Keywords 

HomogeneityMALDIMicro-spottingQuantificationReproducibility

Abstract 

Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.

ISSN 

1044-0305

Link 

http://dx.doi.org/10.1007/s13361-014-0919-7

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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