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Title 

Platycodon grandiflorum polysaccharide induces dendritic cell maturation via TLR4 signaling

Authors 

M J ParkH S RyuJ S KimH K LeeJong Soon KangJi Eun YunS Y KimM K LeeJ T HongY KimS B Han

Publisher 

Elsevier

Issue Date 

2014

Citation 

Food and Chemical Toxicology, vol. 72, no. C, pp. 212-220

Keywords 

Dendritic cellMAPKNF-κBPlatycodon grandiflorumTLR4

Abstract 

Dendritic cell (DC) maturation is critical for initiation of the adoptive immune response. DC maturation is often attenuated in several pathological conditions including cancer. In this study, we report the effect of Platycodon grandiflorum polysaccharide (PG) on DC maturation. PG induced phenotypic maturation of DCs, as proved by the increase in the expression of CD40, CD80, CD86, and major histocompatibility complex (MHC)-I/II on the cell surface. PG also induced functional maturation of DCs, as proved by elevated production of interleukin (IL)-12, tumor necrosis factor-α, IL-1β, IL-6, IL-10, and interferon-β, and by enhanced allogeneic T cell stimulation ability of PG-treated DCs. PG efficiently induced maturation of DCs from C3H/HeN mice, which have normal Toll-like receptor-4 (TLR4), but not that of DCs from C3H/HeJ mice, which have mutated TLR4, suggesting that TLR4 might be one of the PG receptors in DCs. In line with TLR4 activation, PG increased the phosphorylation of ERK, p38, and JNK, and the nuclear translocation of p-c-Jun, p-CREB, and c-Fos. PG also activated NF-κB signaling, as evidenced by degradation of IκBα/β and nuclear translocation of p65 and p50. In summary, our data suggest that PG induces DC maturation by activating MAPK and NF-κB signaling downstream of TLR4.

ISSN 

0278-6915

Link 

http://dx.doi.org/10.1016/j.fct.2014.07.011

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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