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Title 

Inactivation of the phosphoglucomutase gene pgm in Paenibacillus polymyxa leads to overproduction of fusaricidin

 

포스포그루코뮤타제 유전자의 불활성화에 의한 패니바실러스 폴리믹사균의 푸자리시딘 생산성 증진

Authors 

Ha Rim KimSoo Young ParkSeong-Bin KimHaeyoung JeongSoo Keun ChoiSeung-Hwan Park

Publisher 

Springer

Issue Date 

2014

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 41, no. 9, pp. 1405-1414

Keywords 

FusaricidinPaenibacillus polymyxapgm mutationPhosphoglucomutase

Abstract 

Fusaricidin, a lipodepsipeptide isolated from Paenibacillus polymyxa, has high antimicrobial activity against fungi and Gram-positive bacteria. Through mutagenesis, we obtained two mutant strains, N1U7 and N17U7, which produce 6.2- to 7.9-fold more fusaricidin than their parent strain. Causal mutations were identified by whole-genome sequencing, and the two strains each contained at least eleven point mutations, including four common mutations. A mutation in the PPE04441 gene (pgm), encoding an α-phosphoglucomutase, was found to be an important factor in fusaricidin overproduction by complementation experiments. Null mutation of pgm in the parental strain increased fusaricidin production by 5.2-fold. Increased growth and cell viability in stationary phase, reduced exopolysaccharide production, and increased fusA expression were observed in the pgm mutant strains, which might be related to fusaricidin overproduction. This is the first report revealing that PGM deficiency leads to an overproduction of fusaricidin.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1007/s10295-014-1470-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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