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Title 

Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

Authors 

Sun Ha KimJae Cheol JeongYoung Ock AhnHaeng Soon LeeSang Soo Kwak

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Molecular Biology Reports, vol. 41, no. 10, pp. 6957-6966

Keywords 

Heavy metalMetallothioneinPhytoremediationReactive oxygen speciesSweetpotato

Abstract 

Metallothioneins (MTs) are cysteine-rich, low molecular weight, metal-binding proteins that are widely distributed in living organisms. Plants produce metal-chelating proteins such as MTs to overcome the toxic effects of heavy metals. We cloned three MT genes from sweetpotato leaves [Ipomoea batatas (L.) Lam.]. The three IbMT genes were classified according to their cysteine residue alignment into type 1 (IbMT1), type 2 (IbMT2), and type 3 (IbMT3). IbMT1 was the most abundantly transcribed MT. It was predominantly expressed in leaves, roots, and callus. IbMT2 transcript was detected only in stems and fibrous roots, whereas IbMT3 was strongly expressed in leaves and stems. The IbMT expression profiles were investigated in plants exposed to heavy metals and abiotic stresses. The levels of IbMT1 expression were strongly elevated in response to Cd and Fe, and moderately higher in response to Cu. The IbMT3 expression pattern in response to heavy metals was similar to that of IbMT1. Exposure to abiotic stresses such as methyl viologen (MV; paraquat), NaCl, polyethylene glycol (PEG), and H2O2 up-regulated IbMT expression; IbMT1 responded strongly to MV and NaCl, whereas IbMT3 was induced by low temperature and PEG. Transgenic Escherichia coli overexpressing IbMT1 protein exhibited results suggest that IbMT could be a useful tool for engineering plants with enhanced tolerance to environmental stresses and heavy metals.

ISSN 

0301-4851

Link 

http://dx.doi.org/10.1007/s11033-014-3582-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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