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Title 

Flavonoids from Machilus japonica stems and their inhibitory effects on LDL oxidation

Authors 

S J JooH J ParkJ H ParkJ G ChoJi Hyun KangTae Sook JeongH C KangD Y LeeH S KimS Y ByunN I Baek

Publisher 

MDPI AG

Issue Date 

2014

Citation 

International Journal of Molecular Sciences, vol. 15, no. 9, pp. 16418-16429

Keywords 

Diphenyl picryl hydrazinylFlavonoidLow-density lipoprotein-oxidationMachilus japonicaNMR

Abstract 

Stems of Machilus japonica were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was successively extracted with ethyl acetate (EtOAc), normal butanol (n-BuOH), and water. Six flavonoids were isolated from the EtOAc fraction: (+)-taxifolin, afzelin, (?)-epicatechin, 5,3'-di-O-methyl-(?)-epicatechin, 5,7,3'-tri-O-methyl-(?)-epicatechin, and 5,7-di-O-methyl-3',4'-methylenedioxyflavan-3-ol. The chemical structures were identified using spectroscopic data including NMR, mass spectrometry and infrared spectroscopy. This is the first report of isolation of these six compounds from M. japonica. The compounds were evaluated for their diphenyl picryl hydrazinyl scavenging activity and inhibitory effects on low-density lipoprotein oxidation. Compounds 1 and 3?6 exhibited DPPH antioxidant activity equivalent with that of ascorbic acid, with half maximal inhibitory concentration (IC50) values of 0.16, 0.21, 0.17, 0.15 and 0.07 mM, respectively. The activity of compound 1 was similar to the positive control butylated hydroxytoluene, which had an IC50value of 1.9 μM, while compounds 3 and 5 showed little activity. Compounds 1, 3, and 5 exhibited LDL antioxidant activity with IC50values of 2.8, 7.1, and 4.6 μM, respectively.

ISSN 

1422-0067

Link 

http://dx.doi.org/10.3390/ijms150916418

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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