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Title 

Toxic pyrene metabolism in Mycobacterium gilvum PYR-GCK results in the expression of mammalian cell entry genes as revealed by transcriptomics study

Authors 

A C BadejoWon Hyung JungNamshin KimS K KimJ C ChaiY S LeeK H JungH J KimY G Chai

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2014

Citation 

Journal of Microbiology and Biotechnology, vol. 24, no. 9, pp. 1170-1177

Keywords 

Mammalian cell entry (mce) genesMycobacteriaPyrene

Abstract 

Mycobacterium gilvum PYR-GCK is a bacterial strain under study for its bioremediation use on heavy hydrocarbon pollutants in the environment. During the course of our study, mammalian cell entry (mce) genes, known to facilitate pathogenicity in M. tuberculosis, were highly expressed during a comparative and substrate-related cultural global transcriptomic study. RNA sequencing of the global transcriptome of the test strain in two different substrates, pyrene and glucose, showed high expression of the mce genes based on the differential results. After validating the expression of these genes with quantitative real-time PCR, we arrived at the conclusion that the genes were expressed based on the pyrene substrate (a phytosterol compound), and sterol metabolism is said to activate the expression of the mce genes in some actinomycetes bacteria, M. gilvum PYR-GCK in this case. This study is believed to be important based on the fact that some mycobacterial strains are undergoing a continuous research as a result of their use in practical bioremediation of anthropogenic exposure of toxic organic wastes in the environment. --------------------------------------------------------------------------------

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1311.11101

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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