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Title 

Purification and functional characterization of the first stilbene glucoside-specific beta-glucosidase isolated from Lactobacillus kimchi

 

락토바실러스 유래 균으로부터 새로운 스틸벤 배당화합물의 분리 및 기능성 연구

Authors 

Jin-A KoJi-Young ParkHyung Jun KwonYoung Bae RyuHyung Jae JeongSu-Jin ParkCha Young KimHyun-Mee OhChan Sun ParkY H LimD KimMun Chual RhoWoo Song LeeYoung-Min Kim

Publisher 

Elsevier

Issue Date 

2014

Citation 

Enzyme and Microbial Technology, vol. 67, no. 0, pp. 59-66

Keywords 

BioconversionLactobacillus kimchiPolydatinResveratrolβ-Glucosidase

Abstract 

This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydatin. This enzyme showed high substrate specificities towards different substrates in the following order: isorhaponticin "polydatin" mulberroside A>oxyresveratrol-3-O-glucoside. Additionally, it rarely hydrolyzed astringin and desoxyrhaponticin. Based on these catalytic specificities, we suggest this enzyme be named stilbene glucoside-specific β-glucosidase. Furthermore, polydatin extracts from Polygonum cuspidatum were successfully converted to resveratrol with a high yield (of over 99%). Stilbene glucoside-specific β-glucosidase is the first enzyme isolated from lactic acid bacteria capable of bio-converting various stilbene glucosides into stilbene.

ISSN 

0141-0229

Link 

http://dx.doi.org/10.1016/j.enzmictec.2014.09.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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