상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Identification of DNA aptamers toward epithelial cell adhesion molecule via Cell-SELEX

Authors 

Ji Won KimEun Young KimSun Young KimSang Kyung ByunDasom LeeKyoung Jin OhWon Kon KimBaek Soo HanSeung-Wook ChiSang Chul LeeKwang-Hee Bae

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Molecules and Cells, vol. 37, no. 10, pp. 742-746

Keywords 

aptamerEpCAMSELEXstem cell marker

Abstract 

The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.14348/molcells.2014.0208

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)