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Title 

Neuraminidase inhibitory activities of quaternary isoquinoline alkaloids from Corydalis turtschaninovii rhizome

 

현호색으로부터 분리되어진 4차 이소퀴놀린 알카로이드계 화합물의 뉴라미니데이즈 저해활성

Authors 

Jang Hoon KimYoung Bae RyuWoo Song LeeYoung Ho Kim

Publisher 

Elsevier

Issue Date 

2014

Citation 

Bioorganic & Medicinal Chemistry, vol. 22, no. 21, pp. 6047-6052

Keywords 

Bacterial neuraminidaseCorydalis turtschaninoviiFumariaceaeIsoquinoline alkaloidsNon-competitive inhibition

Abstract 

Clostridium perfringens is a Gram-positive spore-forming bacterium that causes food poisoning. The neuraminidase (NA) protein of C. perfringens plays a pivotal role in bacterial proliferation and is considered a novel antibacterial drug target. Based on screens for novel NA inhibitors, a 95% EtOH extract of Corydalis turtschaninovii rhizome showed NA inhibitory activity (68% at 30 μg/ml), which resulted in the isolation of 10 isoquinoline alkaloids; namely, palmatine (1), berberine (2), coptisine (3), pseudodehydrocorydaline (4), jatrorrhizine (5), dehydrocorybulbine (6), pseudocoptisine (7), glaucine (8), corydaline (9) and tetrahydrocoptisine (10). Interestingly, seven quaternary isoquinoline alkaloids 1-7 (IC50 = 12.8 ± 1.5 to 65.2 ± 4.5 μM) showed stronger NA inhibitory activity than the tertiary alkaloids 8-10. In addition, highly active compounds 1 and 2 showed reversible non-competitive behavior based on a kinetic study. Molecular docking simulations using the Autodock 4.2 software increased our understanding of receptor-ligand binding of these compounds. In addition, we demonstrated that compounds 1 and 2 suppressed bacterial growth.

ISSN 

0968-0896

Link 

http://dx.doi.org/10.1016/j.bmc.2014.09.004

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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