상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Characterization of a lichenase isolated from soil metagenome

 

메타게놈유래 리치네이즈

Authors 

Sang Yoon KimDoo-Byoung OhOh Suk Kwon

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2014

Citation 

Journal of Microbiology and Biotechnology, vol. 24, no. 12, pp. 1699-1706

Keywords 

Endo-β-1,3-1,4-glucanaseGlycosyl hydrolase family 8LichenaseRecombinant proteinSoil metagenome

Abstract 

A lichenase gene (mt-lic) was identified for the first time through function-based screening of a soil metagenomic library. Its deduced amino acid sequence exhibited a high degree of homology with endo-β-1,3-1,4-glucanase having both lichenase and chitosanase activities, encoded by the bgc gene of Bacillus circulans WL-12. The recombinant lichenase overexpressed and purified from Escherichia coli was able to efficiently hydrolyze both barley β-glucan and lichenan. The enzyme showed maximal activity at a pH of 6.0 at 50°C, with Azo-barley-glucan as the substrate. The metal ions Mn2+, Mg2+, Ca2+, and Fe2+ enhanced the enzymatic activity, whereas the Cu2+ and Zn2+ ions inhibited the enzymatic activity. The Km and Vmax values of the purified lichenase were determined to be 0.45 mg/ml and 24.83 U/min per mg of protein, respectively

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1406.06012

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)