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Title 

Imidazolized magnetic nanovectors with endosome disrupting moieties for the intracellular delivery and imaging of siRNA

Authors 

E KimH LeeY AnE JangEun Kyung LimB KangJ S SuhY M HuhS Haam

Publisher 

Royal Society of Chemistry

Issue Date 

2014

Citation 

Journal of Materials Chemistry, vol. 2, no. 0, pp. 8566-8575

Abstract 

The development of a synchronized delivery and imaging system for small interfering RNA (siRNA) is required for the clinical application of RNA interference (RNAi) in cancer treatment. Herein, we report a pH-responsive, magnetic nanoparticle-based siRNA delivery system that can facilitate the safe and efficient delivery and visualization of therapeutic siRNA by high-resolution magnetic resonance (MR) imaging. Cationic poly-l-lysine-graft-imidazole (PLI) with a reactive silane moiety was stably immobilized onto the surface of the assembled manganese ferrite nanoparticles (MFs) through an emulsion process, ensuring high water solubility, enhanced MR contrast effect, and endosome-disrupting functionality. The synthesized nanovectors were then complexed with siRNA targeting the CD44 gene via electrostatic interactions to verify the specific gene-silencing effect. The imidazolized magnetic nanovector (ImMNV) architectures developed here facilitated improved cellular internalization and exhibited a high level in vitro downregulation compared to non-imidazolized MNVs in metastatic breast cancer cells.

ISSN 

0959-9428

Link 

http://dx.doi.org/10.1039/c4tb01274g

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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