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Title 

Enzymatic biosynthesis of novel resveratrol glucoside and glycoside derivatives

Authors 

R P PandeyP ParajuliJ Y ShinJ LeeS LeeYoung-Soo HongY I ParkJoong Su KimJ K Sohng

Publisher 

American Society for Microbiology

Issue Date 

2014

Citation 

Applied and Environmental Microbiology, vol. 80, no. 23, pp. 7235-7243

Abstract 

A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) D- and L-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resveratrol was achieved with α-D-glucose as the sugar donor to produce four different glucosides of resveratrol: resveratrol 3-O-β-D-glucoside, resveratrol 4'-O-β-D-glucoside, resveratrol 3,5- O-β-D-diglucoside, and resveratrol 3,5,4'-O-β-D-triglucoside. The conversion rates and numbers of products formed were found to vary with the other NDP sugar donors. Resveratrol 3-O-β-D-2-deoxyglucoside and resveratrol 3,5-O-β-D-di-2-deoxyglucoside were found to be produced using TDP-2-deoxyglucose as a donor; however, the monoglycosides resveratrol 4'-O-β-D-galactoside, resveratrol 4'-O-β-D-viosaminoside, resveratrol 3-O-β-L-rhamnoside, and resveratrol 3-O-β-L-fucoside were produced from the respective sugar donors. Altogether, 10 diverse glycoside derivatives of the medically important resveratrol were generated, demonstrating the capacity of YjiC to produce structurally diverse resveratrol glycosides.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.02076-14

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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