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Title 

Characterization of glutamate decarboxylase from Lactobacillus plantarum and its C-terminal function for the pH dependence of activity

Authors 

S M ShinH KimY JooS J LeeY J LeeSang Jun LeeD W Lee

Publisher 

American Chemical Society

Issue Date 

2014

Citation 

Journal of Agricultural and Food Chemistry, vol. 62, no. 50, pp. 12186-12193

Keywords 

C-terminal regionGABAGlutamate decarboxylaseLactobacillus plantarumPH dependence

Abstract 

The gadB gene encoding glutamate decarboxylase (GAD) from Lactobacillus plantarum was cloned and expressed in Escherichia coli. The recombinant enzyme exhibited maximal activity at 40 °C and pH 5.0. The 3D model structure of L. plantarum GAD proposed that its C-terminal region (Ile454-Thr468) may play an important role in the pH dependence of catalysis. Accordingly, C-terminally truncated (Δ3 and Δ11 residues) mutants were generated and their enzyme activities compared with that of the wild-type enzyme at different pH values. Unlike the wild-type GAD, the mutants showed pronounced catalytic activity in a broad pH range of 4.0-8.0, suggesting that the C-terminal region is involved in the pH dependence of GAD activity. Therefore, this study may provide effective target regions for engineering pH dependence of GAD activity, thereby meeting industrial demands for the production of γ-aminobutyrate in a broad range of pH values.

ISSN 

0021-8561

Link 

http://dx.doi.org/10.1021/jf504656h

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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