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Title 

Evaluation of diethylnitrosamine-or hepatitis B virus X gene-induced hepatocellular carcinoma with 18F-FDG PET/CT: a preclinical study

Authors 

J H ParkJ H KangY J LeeK I KimT S LeeK M KimJ A ParkY O KoDae Yeul YuS S NahmT J JeonY S ParkS M Lim

Publisher 

Spandidos Publications

Issue Date 

2015

Citation 

Oncology Reports, vol. 33, no. 1, pp. 347-353

Keywords 

18F-FDG-PET/CTDiethylnitrosamineHepatitis B virus X proteinHepatocellular carcinomaTumor grading

Abstract 

The aim of this study was to evaluate whether the development of hepatocellular carcinoma (HCC) in murine models resembles tumor progression in humans, using non-invasive molecular imaging methods. Murine HCC models were generated by treating mice with diethylnitrosamine (DEN) or by the transgenic expression of hepatitis B virus X (HBx) protein (HBx-Tg model). Tumor development was detected using 18F-fluoro-2-deoxyglucose (18F-FDG) positron emission tomography (PET)/computed tomography (CT) and magnetic resonance imaging (MRI). The histopathological changes and expression of glucose transporter 1 (Glut1) and hexokinase 2 (HK2) were evaluated using hematoxylin and eosin and immunohistochemical staining, respectively. Tumor lesions as small as 1 mm in diameter were detected by MRI. Tumor development was monitored using 18F-FDG PET/CT at 6.5-10 months after DEN treatment or 11-20 months after birth of the HBx-Tg model mice. A correlation study between the 18F-FDG uptake levels and expression levels of HK2 and Glut1 in developed HCC showed a high 18F-FDG uptake in poorly differentiated HCCs that expressed high levels of HK2, in contrast to that in well-differentiated tumors. The progression of primary HCCs resembling human HCC in murine models was detected and monitored by 18F-FDG PET/CT. The correlation between tumor size and SUVmax was verified in the two HCC models. To the best of our knowledge, this is the first study to demonstrate that in vivo 18F-FDG uptake varies in HCCs according to differentiation grade in a preclinical study.

ISSN 

1021-335X

Link 

http://dx.doi.org/10.3892/or.2014.3575

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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