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Title 

Characterization of the wzc gene from Pantoea sp. strain PPE7 and its influence on extracellular polysaccharide production and virulence on Pleurotus eryngii

Authors 

M K KimY H LeeHyeran KimJeongyeo LeeJ S Ryu

Publisher 

Elsevier

Issue Date 

2015

Citation 

Microbiological Research, vol. 170, no. 0, pp. 157-167

Keywords 

Extracellular polysaccharidesPleurotus eryngiiProtein-tyrosine kinaseWzc gene

Abstract 

To characterize of the pathogenicity gene from the soft rot pathogen Pantoea sp. PPE7 in Pleurotus eryngii, we constructed over 10,000 kanamycin-resistant transposon mutants of Pantoea sp. strain PPE7 by transposon mutagenesis. One mutant, Pantoea sp. NPPE9535, did not cause a soft rot disease on Pleurotus eryngii was confirmed by the pathogenicity test.The transposon was inserted into the wzc gene and the disruption of the wzc gene resulted in the reduction of polysaccharide production and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. Analysis of the hydropathic profile of this protein indicated that it is composed of two main domains: an N-terminal domain including two transmembrane α-helices and a C-terminal cytoplasmic domain consisting of a tyrosine-rich region. Comparative analysis indicated that the amino acid sequence of Wzc is similar to that of a number of proteins involved in the synthesis or export of polysaccharides in other bacterial sp.cies. Purified GST-Wzc was found to affect the phosphorylation of tyrosine residue in vivo. These results showed that the wzc gene might play an important role in the virulence of Pantoea sp. strain PPE7 in P. eryngii.

ISSN 

0944-5013

Link 

http://dx.doi.org/10.1016/j.micres.2014.08.002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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