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Title 

Identification of a pollen-specific gene, SlCRK1 (RFK2) in tomato

Authors 

Woong Bom KimSo Young YiS K OhChan Ju LimHyun A KimHyun A JangHa Yeon LeeY I ParkSuk Yoon Kwon

Publisher 

The Genetics Society of Korea

Issue Date 

2014

Citation 

Genes & Genomics, vol. 36, no. 3, pp. 303-311

Keywords 

ArabidopsisPollenPromoterSlCRK1Tissue-specific geneTomato

Abstract 

Plant receptor-like kinases (RLKs) are proteins that are involved in the regulation of development, hormone signaling, abiotic, and biotic stress responses. It has been suggested that cysteine-rich receptor-like kinases (CRKs), which are one of the largest RLK groups, is significant in pathogen defense and programmed cell death. The CRK1 gene is isolated and characterized from tomato (Solanum lycopersicum L.). The SlCRK1 has two C-X8-C-X2-C motifs: a trans-membrane region and a kinase domain similar to other CRKs. The semi-quantitative RT-PCR exhibits the specific expression of SlCRK1 in the flower, but not in the root, leaf, seed, and fruit of the tomato. In addition, SlCRK1 exhibits pollen-specific expression in the floral organ. SlCRK1 has pollen-specific cis-acting elements in the promoter region, and its promoter has pollen-specific activity in the homozygous transgenic plants of tomato and Arabidopsis as confirmed through histochemical GUS assays. Moreover, the expression of SlCRK1 is not detected via stress treatment or hormone treatment. In this study, SlCRK1 from tomato is characterized and its promoter can be useful in developing transgenic plants with foreign genes that should be expressed in pollens.

ISSN 

0254-5934

Link 

http://dx.doi.org/10.1007/s13258-013-0168-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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