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Title 

Improvement of fermentative production of exopolysaccharides from Aureobasidium pullulans under various conditions

Authors 

C SeoHong-Weon LeeA SureshJ W YangJoon Ki JungY C Kim

Publisher 

Korean Institute of Chemical Engineer

Issue Date 

2014

Citation 

Korean Journal of Chemical Engineering, vol. 31, no. 8, pp. 1433-1437

Keywords 

AgitationExopolysaccharideFermentationNutrientsOptimization

Abstract 

The optimization of exopolysaccharide (EPS) production was investigated in the polymorphic fungal strain of Aureobasidium pullulans (KCTC 6081) with varying pH, nutrients concentration, and mixing parameters in batch fermentation condition. The maximum production of EPS (∼7.5 g/L) was observed at pH 4, while optimum nutrient concentration of carbon (sucrose), nitrogen (NaNO3), phosphorous (K2HPO4), and ascorbic acid was 50 g/L, 5 g/L, 1 g/L and 2 g/L, respectively. Interestingly, EPS productivity under non pH controlled fermentation conditions was 0.12 g/L/h with 400 rpm mixing, while under a controlled pH of 4, the EPS productivity was 0.21 g/L/h with 600 rpm, respectively. The fed-batch fermentation increased the EPS productivity up to 0.345 g/L/h with changing mixing conditions from 200 to 600 rpm and reached 47 g/L with 88% pullulan. Thus, pH and mixing were the key parameters for enhancing EPS production from A. pullulans. It is expected that these optimized parameters can be well used for enhanced industrial production of pullulan.

ISSN 

0256-1115

Link 

http://dx.doi.org/10.1007/s11814-014-0064-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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