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Title 

Global transcriptome profiling of genes that are differentially regulated during differentiation of mouse embryonic neural stem cells into astrocytes

Authors 

D HanM R ChoiK H JungNamshin KimS K KimJ C ChaiY S LeeY G Chai

Publisher 

Humana Press

Issue Date 

2015

Citation 

Journal of Molecular Neuroscience, vol. 55, no. 1, pp. 109-125

Keywords 

AstrocytesCell differentiationNeural stem cellsTGF betaWNTs

Abstract 

Many genes are associated with the differentiation of neural stem cells (NSCs) into astrocytes, the most abundant and functionally diverse population of glial cells in the central nervous system, particularly in the brain. In the present study, we differentiated NSCs from the forebrain of embryonic day 14.5 mouse embryos into astrocytes over 1 and 7 days. We identified transcriptomes of NSCs and astrocytes using RNA sequencing and analyzed enriched gene networks, signal pathways, and ontology. To identify important regulators of differentiation, we performed gene clustering according to expression patterns and promoter CG types. Our data show that genes related to system development, including Fbln2, Bcan, Ncam1, Itih3, Tnr, and Vcan, regulate NSC differentiation through WNT/beta-catenin and epithelial to mesenchymal transition pathways. We identified many CG-rich promoter genes related to basic cellular maintenance such as transcription, translation, and structural components and CG-poor promoter genes that are highly associated with cell-type-specific functions or play important roles during development. Our study provides a foundation for further research on NSC differentiation and the future application of stem cells.

ISSN 

0895-8696

Link 

http://dx.doi.org/10.1007/s12031-014-0382-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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