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Title 

Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen-antibody complex structure

Authors 

J H KimP GriponF BouezzedineM S JeongSeung-Wook ChiS E RyuH J Hong

Publisher 

Elsevier

Issue Date 

2015

Citation 

FEBS Letters, vol. 589, no. 2, pp. 193-200

Keywords 

Affinity maturationHepatitis B virusHumanized antibodypreS1Specificity-determining residue graftingVirus neutralization

Abstract 

To improve a previously constructed broadly neutralizing hepatitis B virus (HBV)-specific preS1 humanized antibody (HzKR127), we further humanized it through specificity-determining residue (SDR) grafting. Moreover, we improved affinity by mutating two residues in heavy-chain complementarity-determining regions (CDR), on the basis of the crystal structure of the antigen-antibody complex. HzKR127-3.2 exhibited 2.5-fold higher affinity and enhanced virus-neutralizing activity compared to the original KR127 antibody and showed less immunogenic potential than HzKR127. Enhanced virus-neutralizing activity was achieved by the increased association rate, providing insights into engineering potent antibody therapeutics for HBV immunoprophylaxis. HzKR127-3.2 may be a good candidate for HBV immunoprophylaxis. ? 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/j.febslet.2014.11.046

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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