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Title 

Synergistic effect of two E2 ubiquitin conjugating enzymes in SCF(hFBH1) catalyzed polyubiquitination

Authors 

Jeong Hoon KimJin Sun ChoiSunhong KimKidae KimP K MyungSung Goo ParkY S SeoByoung Chul Park

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2015

Citation 

BMB Reports, vol. 48, no. 1, pp. 25-29

Keywords 

E2 ubiquitin conjugating enzymehFBH1PolyubiquitinationSCFUbiquitin

Abstract 

Ubiquitination is a post translational modification which mostly links with proteasome dependent protein degradation. This process has been known to play pivotal roles in the number of biological events including apoptosis, cell signaling, transcription and translation. Although the process of ubiquitination has been studied extensively, the mechanism of polyubiquitination by multi protein E3 ubiquitin ligase, SCF complex remains elusive. In the present study, we identified UbcH5a as a novel stimulating factor for poly-ubiquitination catalyzed by SCFhFBH1 using biochemical fractionations and MALDI-TOF. Moreover, we showed that recombinant UbcH5a and Cdc34 synergistically stimulate SCFhFBH1 catalyzed polyubiquitination in vitro. These data may provide an important cue to understand the mechanism how the SCF complex efficiently polyubiquitinates target substrates.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2015.48.1.057

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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