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Title 

Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

Authors 

In Yeong KangJang Min ParkWon-Kyung HongYun Seok KimYou Ree JungSeung Bum KimSun-Yeon HeoSung Mok LeeJi Young KangBaek Rock OhDae-Hyuk KimJeong-Woo SeoChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2015

Citation 

Bioprocess and Biosystems Engineering, vol. 38, no. 2, pp. 299-305

Keywords 

2,3-butanediol2,3-butanediol dehydrogenaseBacillus sp.Empty palm fruit bunches

Abstract 

A Bacillus sp.cies that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this sp.cies was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-014-1268-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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