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Title 

Methylation and expression changes in imprinted genes H19 and Igf2 during serial somatic cell nuclear transfer using piglet fibroblasts

Authors 

Hoon JangW G JangEun Jung KimMinhwa DoK B OhS HwangH ShimY G ChooD J KwonJeong Woong Lee

Publisher 

Zoological Society of Korea

Issue Date 

2015

Citation 

Animal Cells and Systems, vol. 19, no. 1, pp. 46-53

Keywords 

bisulfite sequencingepigeneticsimprinted genemini-pigSCNT

Abstract 

Cloned pigs produced by somatic cell nuclear transfer (SCNT) are important as a potential alternative source of organs. Although SCNT has created new possibilities for targeted gene modification, the successful cloning of pigs is rare. Here, we successfully conducted serial SCNT for three generations. We determined that the piglet genome was inherited from donor cell nuclei using microsatellite analysis of each generation. The methylation of differentially methylated regions (DMRs) in H19 was gradually reduced over the three generations of serial SCNT. By contrast, methylation of the insulin-like growth factor 2 (Igf2) DMR increased in the F1 generation, compared to the F0, and remained at the higher level in the F2 and F3 generations. The methylation patterns of housekeeping genes such as GAPDH and β-actin were unchanged in the serially cloned pigs. In addition, expression levels of H19 and Igf2 were variable for each generation of serial SCNT piglets, but there was no clear relationship between the methylation and gene expression patterns. Our study conclusively demonstrates that the methylation patterns of DMRs in H19 and Igf2 were altered, compare to the F0 donor, during serial SCNT, but housekeeping genes were unaffected.

ISSN 

1976-8354

Link 

http://dx.doi.org/10.1080/19768354.2014.995706

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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