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Title 

Assessment of difference in gene expression profile between embryos of different derivations

Authors 

Sujin KwonS JeongYoung Sun JeongJung Sun ParkX S CuiN H KimYong-Kook Kang

Publisher 

Mary Ann Liebert

Issue Date 

2015

Citation 

Cellular Reprogramming, vol. 17, no. 1, pp. 49-58

Abstract 

Researchers have exerted sustained efforts to improve the viability of somatic cell nuclear transfer (SCNT) embryos, testing their experimental designs and probing the resultant embryos. However, the lack of a reliable method to estimate the efficacy of these experimental attempts is a chief hindrance to tackling the low-viability problem in SCNT. Here, we introduce a procedure that assesses the degree of difference in gene expression profiles (GEPs) of blastocysts from each other as a representative control of good quality. We first adapted a multiplex reverse transcription-polymerase chain reaction strategy to obtain GEPs for 15 reprogramming-related genes from single mouse blastocysts. GEPs of individual blastocysts displayed a broad range of variations, the extent of which was calculated using a weighted root mean square deviation (wRMSD). wRMSD-based quantitation of GEP difference (qGEP) found that GEP difference between in vivo - derived blastocysts (in vivo) and SCNT blastocysts was greater than the difference between in vivo blastocysts and in vitro - produced (IVP) blastocysts, demonstrating that the SCNT group was more distantly related to the in vivo group than the IVP group. Our qGEP approach for grading individual blastocysts would be useful for selecting a better protocol to derive embryos of better quality prior to field applications.

ISSN 

2152-4971

Link 

http://dx.doi.org/10.1089/cell.2014.0057

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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