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Title 

Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1

Authors 

S M BongS W YangJ W ChoiSeung Jun KimB I Lee

Publisher 

International Union of Crystallography

Issue Date 

2015

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 71, no. 3, pp. 261-265

Keywords 

MTMRmyotubularinphosphatasephosphatidylinositol

Abstract 

Myotubularin-related protein 1 is a phosphatase that dephosphorylates phospholipids such as phosphatidylinositol 3-phosphate or phosphatidylinositol 3,5-bisphosphate. In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277K using polyethylene glycol 20000 as a precipitant. Diffraction data were collected to 2.0? resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.581?, α = 87.597, β = 86.072, γ = 77.327°. Assuming the presence of four molecules in the asymmetric unit, the calculated Matthews coefficient value was 2.61?3Da-1 and the corresponding solvent content was 52.9%.

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 71(3): 261-265

ISSN 

1744-3091

Link 

http://dx.doi.org/10.1107/S2053230X15000606

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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