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Title | Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels |
Authors | Dae Sub Song; G Ha; W Serhan; Y Eltahir; M Yusof; F Hashem; E Elsayed; B Marzoug; A Abdelazim; S A Muhairi |
Publisher | American Society for Microbiology |
Issue Date | 2015 |
Citation | Journal of Clinical Microbiology, vol. 53, no. 4, pp. 1178-1182 |
Abstract | We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. |
ISSN | 0095-1137 |
Link | |
Appears in Collections | |
Registered Date |
2017-04-19 |
