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Title 

Development and validation of a rapid immunochromatographic assay for detection of middle East respiratory syndrome coronavirus antigen in dromedary camels

Authors 

Dae Sub SongG HaW SerhanY EltahirM YusofF HashemE ElsayedB MarzougA AbdelazimS A Muhairi

Publisher 

American Society for Microbiology

Issue Date 

2015

Citation 

Journal of Clinical Microbiology, vol. 53, no. 4, pp. 1178-1182

Abstract 

We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.

ISSN 

0095-1137

Link 

http://dx.doi.org/10.1128/JCM.03096-14

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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