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Title 

Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs

Authors 

J Y YeonS H MinH J ParkJ W KimY H LeeS Y ParkPil-Soo JeongH ParkD S LeeSun-Uk KimKyu Tae ChangD B Koo

Publisher 

Society for Reproduction and Development

Issue Date 

2015

Citation 

Journal of Reproduction Development, vol. 61, no. 2, pp. 81-89

Keywords 

EmbryosFibroblast cellsMdivi-1MitochondriaPig

Abstract 

Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells. Key words: Embryos, Fibroblast cells, Mdivi-1, Mitochondria, Pig.

ISSN 

0916-8818

Link 

http://dx.doi.org/10.1262/jrd.2014-070

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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