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Title 

Photo-crosslinkable hydrogel-based 3D microfluidic culture device

Authors 

Y LeeJ M LeePan Kee BaeI Y ChungBong Hyun ChungB G Chung

Publisher 

Wiley-Blackwell

Issue Date 

2015

Citation 

Electrophoresis, vol. 36, no. 7, pp. 994-1001

Keywords 

HydrogelMicrofluidic deviceStem cell

Abstract 

We developed the photo-crosslinkable hydrogel-based 3D microfluidic device to culture neural stem cells (NSCs) and tumors. The photo-crosslinkable gelatin methacrylate (GelMA) polymer was used as a physical barrier in the microfluidic device and collagen type I gel was employed to culture NSCs in a 3D manner. We demonstrated that the pore size was inversely proportional to concentrations of GelMA hydrogels, showing the pore sizes of 5 and 25 w/v% GelMA hydrogels were 34 and 4 μm, respectively. It also revealed that the morphology of pores in 5 w/v% GelMA hydrogels was elliptical shape, whereas we observed circular-shaped pores in 25 w/v% GelMA hydrogels. To culture NSCs and tumors in the 3D microfluidic device, we investigated the molecular diffusion properties across GelMA hydrogels, indicating that 25 w/v% GelMA hydrogels inhibited the molecular diffusion for 6 days in the 3D microfluidic device. In contrast, the chemicals were diffused in 5 w/v% GelMA hydrogels. Finally, we cultured NSCs and tumors in the hydrogel-based 3D microfluidic device, showing that 53-75% NSCs differentiated into neurons, while tumors were cultured in the collagen gels. Therefore, this photo-crosslinkable hydrogel-based 3D microfluidic culture device could be a potentially powerful tool for regenerative tissue engineering applications.

ISSN 

0173-0835

Link 

http://dx.doi.org/10.1002/elps.201400465

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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