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Title 

Digital microfluidic approach for efficient electroporation with high productivity: Transgene expression of microalgae without cell wall removal

Authors 

D J ImS N JeongB S YooB KimD P KimWon Joong JeongI S Kang

Publisher 

American Chemical Society

Issue Date 

2015

Citation 

Analytical Chemistry, vol. 87, no. 13, pp. 6592-6599

Abstract 

A unique digital microfluidic electroporation (EP) system successfully demonstrates higher transgene expression than that of conventional techniques, in addition to reliable productivity and feasible integrated processes. By systematic investigations into the effects of the droplet EP conditions for a wild-type microalgae, 1 order of magnitude higher transgene expression is accomplished without cell wall removal over the conventional bulk EP system. In addition, the newly proposed droplet EP method by a droplet contact charging phenomena shows a great potential for the integration of EP processes and on-chip cell culture providing easy controllability of each process. Finally, the implications of the accomplishments and future directions for development of the proposed technology are discussed. (Figure Presented.)

ISSN 

0003-2700

Link 

http://dx.doi.org/10.1021/acs.analchem.5b00725

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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