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Title 

The heat shock protein 70a from Pyropia seriata increases heat tolerance in Chlamydomonas

Authors 

S ChoiS H KimS O ImWon Joong JeongM S HwangE J ParkD W Choi

Publisher 

Southern Cross Publishing

Issue Date 

2015

Citation 

Plant Omics Journal, vol. 8, no. 4, pp. 327-334

Keywords 

Heat shock protein 70Heat tolerancePyropia seriataRed algaeTransgene

Abstract 

The heat shock protein 70 (HSP70) molecular chaperones constitute a large family of highly conserved proteins, which are key survival components under stress and normal physiological conditions. Pyropia seriata grows on intertidal rocks, where it is exposed to environmental changes including desiccation and temperature variations. We identified five HSP70 cDNAs from P. seriata transcriptome. An amino acid sequence analysis suggested that PsHSP70a and PsHSP70d are cytosolic HSP70s, whereas PsHSP70b and PsHSP70c are in the mitochondria and PsHSP70e may be transported into the endoplasmic reticulum. Most of the PsHSP70 genes were detected under normal growth and heat stress conditions, except PsHSP70c. Among them, PsHSP70a displayed the strongest response to heat stress. PsHSP70a-transformed Chlamydomonas showed much higher survival and growth rates than those of wild-type under high temperature conditions. These results indicate that the Pyropia genome contains at least five HSP70 genes, and that cytosolic PsHSP70a is involved in high temperature stress tolerance.

ISSN 

1836-3644

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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