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Title 

A novel and highly specific phage endolysin cell wall binding domain for detection of Bacillus cereus

Authors 

M KongJieun SimTaejoon KangH H NguyenH K ParkBong Hyun ChungS Ryu

Publisher 

Springer Verlag (Germany)

Issue Date 

2015

Citation 

European Biophysics Journal With Biophysics Letters, vol. 44, no. 6, pp. 437-446

Keywords 

Bacillus cereusBacteriophage endolysinBiosensorCell wall binding domainSurface plasmon resonance

Abstract 

Rapid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety. Bacillus cereus is harmful as it causes foodborne illness and a number of systemic and local infections. We report a novel phage endolysin cell wall-binding domain (CBD) for B. cereus and the development of a highly specific and sensitive surface plasmon resonance (SPR)-based B. cereus detection method using the CBD. The newly discovered CBD from endolysin of PBC1, a B. cereus-specific bacteriophage, provides high specificity and binding capacity to B. cereus. By using the CBD-modified SPR chips, B. cereus can be detected at the range of 105?108 CFU/ml. More importantly, the detection limit can be improved to 102 CFU/ml by using a subtractive inhibition assay based on the pre-incubation of B. cereus and CBDs, removal of CBD-bound B. cereus, and SPR detection of the unbound CBDs. The present study suggests that the small and genetically engineered CBDs can be promising biological probes for B. cereus. We anticipate that the CBD-based SPR-sensing methods will be useful for the sensitive, selective, and rapid detection of B. cereus.

ISSN 

0175-7571

Link 

http://dx.doi.org/10.1007/s00249-015-1044-7

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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