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Title 

In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination

Authors 

Hyung Jun KwonJ H JeongS W LeeYoung Bae RyuHyung Jae JeongKyungsook JungJ S LimK O ChoWoo Song LeeMun Chual RhoSu-Jin Park

Publisher 

Japanese Pharmacological Society

Issue Date 

2015

Citation 

Journal of Pharmacological Sciences, vol. 128, no. 4, pp. 159-169

Keywords 

Anti-reovirus activityHemagglutinationKuraridinSophora flavescensViral absorption and replication

Abstract 

In this study, we evaluated the anti-reovirus activity of kuraridin isolated from the roots of Sophora flavescens. In particular, we focused on whether this property is attributable to direct inhibition of reovirus attachment and/or inhibition of viral replication with the aid of time-of-addition (pre-treatment, simultaneous treatment, and post-treatment) experiments. No significant antiviral activity of kuraridin was detected in the pre-treatment assay. In the simultaneous assay, the 50% effective inhibitory concentrations (EC50) of kuraridin were 15.3-176.9 μM against human type 1-3 reoviruses (HRV1-3) and Korean porcine reovirus (PRV). Kuraridin completely blocked binding of viral sigma 1 protein to sialic acids at concentrations lower than 82.5 μM in the hemagglutination inhibition assay. Moreover, kuraridin inhibited HRV1-3 and PRV viral replication with EC50 values of 14.0-62.0 μM. Quantitative real-time PCR analysis disclosed strong suppression of reovirus RNA synthesis at the late stage (18 h) of virus replication by kuraridin. The viral yields of kuraridin-treated cells were significantly reduced at 24 h post-infection, compared with DMSO-treated cells. Our results collectively suggest that kuraridin inhibits virus adsorption and replication by inhibiting hemagglutination, viral RNA and protein synthesis and virus shedding, supporting its utility as a viable candidate antiviral drug against reoviruses.

ISSN 

1347-8613

Link 

http://dx.doi.org/10.1016/j.jphs.2015.04.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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