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Title 

Development of transgenic cassava plants expressing IbOr gene by somatic embryogenesis

Authors 

Sun Ha KimM D KimSung-Chul ParkJae Cheol JeongHaeng Soon LeeSang Soo Kwak

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2015

Citation 

Journal of Plant Biotechnology, vol. 42, no. 2, pp. 88-92

Keywords 

CarotenoidCassavaIbOr geneOxidative stressSWPA2 promoter

Abstract 

Cassava (Manihot esculenta Crantz) is a useful root crop for food, animal feed and various industrial materials including biofuel. Despite of its importance as an industrial crop, the genetic engineering approaches to manipulate transgenic plant development in cassava are limited. In this study, to develop new cultivar with high level of carotenoids and enhanced tolerance to environmental stresses, sweetpotato IbOr gene involved in accumulation of carotenoids was introduced into an Indonesian IDB high-yielding cassava cultivar under the control of oxidative stress-inducible SWPA2 promoter through Agrobacterium-mediated transformation of friable embryogenic calli. The 19 transgenic lines were successfully generated on the basis of gDNA-PCR and IbOr transcript levels for further characterization in terms of carotenoid contents and environmental stresses. Therefore, IbOr transgenic cassava plants may be developed for enhanced biomass production with high levels of carotenoids on marginal lands.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2015.42.2.88

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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