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Title 

Anaerosolibacter carboniphilus gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil

Authors 

H HongS J KimU G MinYong Jae LeeSong-Gun KimS W RohJ G KimJ G NaS K Rhee

Publisher 

Society for General Microbiology

Issue Date 

2015

Citation 

International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 5, pp. 1480-1485

Abstract 

A strictly anaerobic, mesophilic, iron-reducing bacterial strain, IRF19T, was isolated from coal-contaminated soil in the Republic of Korea. IRF19T cells were straight, rod-shaped, Gram-staining-negative and motile by means of flagella. The optimum pH and temperature for their growth were determined to be pH 7.5-8.0 and 40°C, while the optimum range was pH 6.5- 10.0 and 20-45°C, respectively. Strain IRF19T did not require NaCl for growth but it tolerated up to 2 % (w/v). Growth was observed with yeast extract, D-glucose, D-fructose, D-ribose, D-mannitol, D-mannose, L-serine, L-alanine and L-isoleucine. Fe(III), elemental sulfur, thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain IRF19T is affiliated to the family Clostridiaceae and is most closely related to Salimesophilobacter vulgaris Zn2T (93.5 % similarity), Geosporobacter subterraneus VNs68T (93.2 %) and Thermotalea metallivorans B2-1T (92.3 %). The major cellular fatty acids of strain IRF19T were C14 : 0, iso-C15 : 0 and C16 : 0, and the profile was distinct from those of the closely related species. The major respiratory quinone of strain IRF19T was menaquinone MK-5 (V-H2). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain IRF19T was determined to be 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic results, strain IRF19T is considered to represent a novel species of a novel genus of the family Clostridiaceae, for which we propose the name Anaerosolibacter carboniphilus gen. nov., sp. nov., with the type strain IRF19T (=KCTC 15396T=JCM 19988T).

ISSN 

1466-5026

Link 

http://dx.doi.org/10.1099/ijs.0.000124

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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