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Title 

Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers

Authors 

Eun-Yee JieYoung Bae RyuSeung A ChoiMyung-Suk AhnJang Ryol LiuSung Ran MinSuk Weon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2015

Citation 

Plant Biotechnology Reports, vol. 9, no. 5, pp. 331-338

Keywords 

Microtuber productionPinellia ternataPlant regenerationSuccinic acid

Abstract 

The conditions for efficient tuber production from suspension cultures of Pinellia ternata cells which is one of medicinal herbs were established and succinic acid in tubers propagated in vitro was determined. Leaf explants formed white nodular structures and off-white calluses at a frequency of 90.6 % when cultured on Murashige and Skoog medium supplemented with 0.54 μM α-naphthaleneacetic acid (NAA); however, this frequency declined substantially with increasing NAA concentration, up to 16.2 μM, at which the frequency reached zero percent. In combination treatments with 4.44 μM 6-benzyladenine (BA) and NAA, however, the frequency of white nodular structure and off-white callus formation from leaf explants did not decrease, even at 16.2 μM NAA. Suspension cultures of P. ternata cells were established from leaf-derived off-white calluses in MS liquid medium containing 5.4 μM NAA and 4.44 μM BA. Upon plating onto MS basal medium, over 90 % of cell aggregates gave rise to microtubers and developed into plantlets. Regenerated plantlets were transplanted in potting soil and grown to maturity in a growth chamber, with a survival rate of >90 %. The highest succinic acid content in suspension culture-derived microtubers was 45 g/kg of extract. Compared with wild P. ternata medicinal tubers, the succinic acid content was very similar. The in vitro P. ternata microtuber proliferation system established in this study is thus an efficient alternative for the mass production of medicinal tubers.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-015-0369-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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