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Title 

Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli

Authors 

G BakJ LeeS SukD KimJ Y LeeKwang-sun KimB S ChoiY Lee

Publisher 

Nature Publishing Group

Issue Date 

2015

Citation 

Scientific Reports, vol. 5, no. 0, pp. 15287-15287

Abstract 

Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm formation. Interestingly, IS118, yet to be characterized, suppressed all the processes. Our data not only reveal potentially critical functions of individual sRNAs in biofilm formation and other phenotypes but also highlight the unexpected complexity of sRNA-mediated metabolic pathways leading to these processes.

ISSN 

2045-2322

Link 

http://dx.doi.org/10.1038/srep15287

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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