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Title 

Azorella compacta methanolic extract induces apoptosis via activation of mitogen-activated protein kinase

Authors 

Min Hee SungOk-Kyoung KwonSei-Ryang OhJ LeeS H ParkS B HanKyung Seop Ahn

Publisher 

Spandidos Publications

Issue Date 

2015

Citation 

Molecular Medicine Reports, vol. 12, no. 5, pp. 6821-6828

Keywords 

ApoptosisAzorella compactaHL60Intrinsic pathwayMitogen-activated protein kinase

Abstract 

Azorella compacta Phil. (AC) is an alpine medicinal plant used traditionally for antibacterial treatment. Recent studies have revealed that this plant also has anti-diabetic effects, but that it is toxic. The present study investigated the underlying mechanisms of action of AC extract against human leukemia HL60 cells. Apoptosis induction was measured by MTT assay, fluorescence microscopy, DNA fragmentation assay, flow cytometric analysis, reverse transcription quantitative polymerase chain reaction and western blot analyses. It was found that AC extract inhibited the growth of HL60 and other cancer cell lines in a dose-dependent manner. The cytotoxic effects of AC extract on HL60 cells were associated with apoptosis characterized by DNA fragmentation and dose-dependent increases in Annexin V-positive cells, as determined by flow cytometric analysis. AC-extract-induced apoptosis was accompanied by activated/cleaved caspase-3, caspase-9 and poly(adenosine diphosphate-ribose) polymerase (PARP). The increases in apoptosis were also associated with decreases of the apoptosis-inhibitor B-cell lymphoma 2 (Bcl-2), upregulation of pro-apoptotic Bcl-2-associated X (Bax) protein and downregulation of anti-apoptotic Bcl extra large protein. Furthermore, western blot analysis of mitogen-activated protein kinase (MAPK)-associated proteins indicated that treatment with AC extract increased the levels of c-Jun N-terminal kinase, extracellular signal-regulated kinase and p38. In addition, the expression of Bax and cleaved PARP was blocked when AC treatment was performed in the presence of MAPK inhibitors. It was therefore concluded that AC induced apoptosis in human leukemia HL60 cells via an intrinsic pathway controlled through MAPK-associated signaling.

ISSN 

1791-2997

Link 

http://dx.doi.org/10.3892/mmr.2015.4317

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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