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Title 

Pancreatic adenocarcinoma upregulated factor serves as adjuvant by activating dendritic cells through stimulation of TLR4

Authors 

T H KangY S KimSeok Ho KimB YangJ J LeeH J LeeJaemin LeeI D JungH D HanS H LeeS S KohT C WuY M Park

Publisher 

Impact Journals

Issue Date 

2015

Citation 

Oncotarget, vol. 6, no. 29, pp. 27751-27762

Keywords 

AdjuvantsCancer vaccinesDendritic cellsPAUFTLR4

Abstract 

Dendritic cell (DC) based cancer vaccines represent a promising immunotherapeutic strategy against cancer. To enhance the modest immunogenicity of DC vaccines, various adjuvants are often incorporated. Particularly, most of the common adjuvants are derived from bacteria. In the current study, we evaluate the use of a human pancreatic cancer derived protein, pancreatic adenocarcinoma upregulated factor (PAUF), as a novel DC vaccine adjuvant. We show that PAUF can induce activation and maturation of DCs and activate NFkB by stimulating the Tolllike receptor signaling pathway. Furthermore, vaccination with PAUF treated DCs pulsed with E7 or OVA peptides leads to generation of E7 or OVA-specific CD8+ T cells and memory T cells, which correlate with long term tumor protection and antitumor effects against TC-1 and EG.7 tumors in mice. Finally, we demonstrated that PAUF mediated DC activation and immune stimulation are dependent on TLR4. Our data provides evidence supporting PAUF as a promising adjuvant for DC based therapies, which can be applied in conjunction with other cancer therapies. Most importantly, our results serve as a reference for future investigation of human based adjuvants.

ISSN 

1949-2553

Link 

http://dx.doi.org/10.18632/oncotarget.4859

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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