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Title 

The fate of extrachromosomal DNAs in the progeny of plastid-transformed tobacco plants

 

엽록체 형질전환 담배의 후대에서 extrachromosomal DNA의 운명

Authors 

Sung Ran MinS H JungJang Ryol LiuWon Joong Jeong

Publisher 

Springer Verlag (Germany)

Issue Date 

2015

Citation 

Plant Biotechnology Reports, vol. 9, no. 6, pp. 431-442

Keywords 

Extrachromosomal DNAHomologous recombinationPlastid transformationTobacco

Abstract 

Plastid transformation is conducted by homologous recombination. Plastid transformation using a vector-containing promoter and/or terminator sequences homologous to the plastid DNA for transgene expression often results in the generation of unintended extrachromosomal DNA molecules derived from the initial plastid DNA molecule via intramolecular recombination. All the extrachromosomal DNA molecules, including those lacking the ori sequence, found in the T0 plastid-transformed tobacco plants were still detected in the T3 plants. These results suggest that the extrachromosomal DNA molecules are newly generated from the initially transformed plastid DNA that is transmitted to the progeny during each generation. In addition, inward, outward, and overlap extension PCR of the extrachromosomal DNA molecules confirmed that these molecules were circular.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-015-0380-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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