상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Chitosan-mediated non-viral gene delivery with improved serum stability and reduced cytotoxicity

Authors 

Sang-Hyun MinKyung Chan ParkYoung Il Yeom

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Biotechnology and Bioprocess Engineering, vol. 19, no. 6, pp. 1077-1082

Keywords 

chitosancytotoxicitygene deliveryPEIpolyethyleniminepolyplex

Abstract 

Polyethyleneimine (PEI) has been widely used in gene delivery systems because of its outstanding ability to promote DNA condensation and endosome escape. However, its use for the introduction of foreign DNA into host cells is compromised by high cytotoxicity and serum reactivity. In this study we examined the possibility of improving the PEI-mediated gene delivery using the serum-stable and biodegradable polymer, chitosan. Among chitosans of different molecular weights, the 45 kD form exhibited significantly higher gene delivery efficiency than the 15 and 100 kD forms. Using the 45 kDa form of chitosan, we formulated composite PEI/DNA/chitosan (PDC) complexes consisting of the core part where DNA chains were tightly condensed by PEI and the outer layer lined with chitosan, and found that an optimal PDC complex exhibits a significantly higher transfection efficiency in a high serum condition and a lower toxicity than monolithic PEI/DNA (PD) complex. Moreover, addition of chitosan to PEI/DNA complex reduced nonspecific interactions with erythrocytes, which may eventually contribute to improved transfection efficiency in high serum concentrations. These results demonstrate that chitosan coating of PD complex might increase the efficiency of PEI-mediated gene delivery in vivo as well as in vitro.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-014-0450-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)